In This Post we are  providing Chapter-11 Biotechnology: Principles and Processes  NCERT MCQ for Class 12 Biology which will be beneficial for students. These solutions are updated according to 2021-22 syllabus. These MCQS  can be really helpful in the preparation of Board exams and will provide you with a brief knowledge of the chapter.

NCERT MCQ ON BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

1. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

(a) The larger the fragment size, the farther it moves

(b) The smaller the fragment size, the farther it moves

(c) Positively charged fragments move to farther end

(d) Negatively charged fragments do not move

Answer: B

2. A gene whose expression helps to identify transformed cell is known as___________

(a) Selectable marker

(b) Vector

(c) Plasmid

(d) Structural gene

Answer: A

3. Which one of the given options correctly identifies its certain component(s)?

(a) Ori – original restriction enzyme

(b) Rop – Reduced osmotic pressure

(c) Hind I, EcoR I – selectable markers

(d) AmpR, tetR – antibiotic resistance genes

Answer: D

4. There is a restriction endonuclease called EcoRI. What does ‘co part in it stand for?

(a) Coelom

(b) Coli

(c) Colon

(d) Coenzyme

Answer: B

5. Given below is a sample of a portion of DNA strand giving the base sequence on the oppositevstrands?

5’____________GAATTC____________3′

5’____________CTTAAG____________3′

What is so special shown in it?

(a) Replication completed

(b) Deletion mutation

(c) Start codon at the 5′ end

(d) Palindromic sequence of base pairs

Answer: D

6. The DNA fragments separated on an agarose gel can be visualised after staining with__________

(a) Bromophenol blue 

(b) Acetocarmine

(c) Aniline blue

(d) Ethidium bromide

Answer: D

7. Which of the following restriction enzymes produces blunt ends?

(a) Eco RV

(b) Sal I

(c) Hind III

(d) Xho I

Answer: A

8. A foreign DNA and plasmid cut by the me restriction endonuclease can be joined to forma recombinant plasmid using_____________

(a) Eco RI

(b) Taq polymerase

(c) Ligase

(d) Polymerase II

Answer: C

9. Which of the following is not a feature of the plasmid?

(a) Single stranded

(b) Independent replication

(c) Circular structure

(d) Small, circular double-stranded

Answer: A

10. Commonly used vectors for human genome sequencing are_____________

(a) T-DNA

(b) BAC vectors

(c) T/C cloning vector

(d) Expression vector

Answer: B

11. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by_________

(a) Centrifugation

(b) Polymerase Chain Reaction

(c) Electrophoresis

(d) Restriction mapping

Answer: C



12. Biolistics (Gene gun) is suitable for_____________

(a) Disarming pathogen vectors

(b) Iransformation of plant cells

(c) Joining of DNA Vector

(d) DNA Fingerprinting 

Answer: B

13. The correct order of steps in Polymerase Chain Reaction (PCR) is____________

(a) Denaturation, Extension, Annealing

(b) Annealing, Extension, Denaturation

(c) Extension, Denaturation, Annealing

(d) Denaturation, Annealing, Extension

Answer: D

14. The process of separation and purification of expressed protein before marketing is called__________

(a) Upstream processing

(b) Downstream processing

(c) Bioprocessing

(d) Postproduction processing

Answer: B

15. Which of the following is not a component of downstream processing? 

(a) Separation

(b) Preservation

(c) Purification

(d) Expression

Answer: D

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