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Exercises
1. By looking at a plant externally can you tell whether a plant is C3 or C4? Why and how?
Answer
We can’t tell whether a plant is C3 or C4 by looking at a plant externally. However, plants which which are adapted to dry climates follow the C4 pathway. Unlike C3 plants, the leaves of C4 plants have a special anatomy but this difference can only be observed at the cellular level.
2. By looking at which internal structure of a plant can you tell whether a plant is C3 or C4? Explain.
Answer
As leaves of C4 plants have a special anatomy called Kranz anatomy. This makes them different from C3 plants. Special cells, known as bundle-sheath cells, surround the vascular bundles. These cells have a large number of chloroplasts. They are thick-walled and have no intercellular spaces. Therefore, we can tell whether a plant is C3 or C4 through internal structure.
3. Even though a very few cells in a C4 plant carry out the biosynthetic – Calvin pathway, yet they are highly productive. Can you discuss why?
AnswerIn C4 plants photorespiration does not occur because they have a mechanism that increases the concentration of CO2 at the enzyme site. This takes place when the C4 acid from the mesophyll is broken down in the bundle sheath cells to release CO2 that results in increasing the intracellular concentration of CO2. In turn, this ensures that the RuBisCO functions as a carboxylase minimising the oxygenase activity. Thus, the photosynthesis rate increases and make C4 plants more productive.
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4. RuBisCo is an enzyme that acts both as a carboxylase and oxygenase. Why do you think RuBisCo carries out more carboxylation in C4 plants?
Answer
The enzyme RuBisCo is absent from the mesophyll cells of C
4 plants. It is present in the bundle-sheath cells surrounding the vascular bundles. In C
4 plants, the Calvin cycle occurs in the bundle-sheath cells. The primary CO
2 acceptor in the mesophyll cells is phosphoenol pyruvate -a three-carbon compound. It is converted into the four-carbon compound oxaloacetic acid (OAA). OAA is further converted into malic acid. Malic acid is transported to the bundle-sheath cells, where it undergoes decarboxylation and CO
2 fixation occurs by the Calvin cycle. This prevents the enzyme RuBisCo from acting as an oxygenase.
5. Suppose there were plants that had a high concentration of Chlorophyll b, but lacked chlorophyll a, would it carry out photosynthesis? Then why do plants have chlorophyll b and other accessory pigments?
Answer
If there were complete absence of chlorophyll a in a plant, it would not carry out photosynthetic activity at all because chlorophyll
a is the chief pigment associated with photosynthesis as it traps light. Other accessory pigments like chlorophyll
b, santhophylls and carotenoids are equally essential as they also absorb light and transfer energy to chlorophyll
a. They also enable a wider range of wavelength of incoming light to be utilised for photosynthesis and protect chlorophyll a from photo- oxidation.
6. Why is the colour of a leaf kept in the dark frequently yellow, or pale green? Which pigment do you think is more stable?
Answer
Chlorophyll or green pigment is unable to absorb energy in the absence of light therefore loses its stability. Thus, the colour of leaf changes to yellow or pale green. This shows that Carotenoids and Xanthophyll pigments are more stable.
7. Look at leaves of the same plant on the shady side and compare it with the leaves on the sunny side. Or, compare the potted plants kept in the sunlight with those in the shade. Which of them has leaves that are darker green? Why?
Answer